Biomedical Engineering Reference
In-Depth Information
Figure 10.12
SEM image of carbon iber (100 nm) compact (magniica-
tion 5000×) [15].
They found that the number of osteoblasts increases on nano-
phase carbon iber compacts (Fig. 10.13) [15]. Compared to conven-
tional compacts, alkaline phosphatase activity was signiicantly
greater when osteoblasts were cultured on respective outer pyrolytic
layer nanophase carbon iber compacts (Fig. 10.14). For any time
period tested, calcium content in the extracellular matrix increases
with decreasing carbon iber diameter (Fig. 10.15). Osteoblast
proliferation increases with decreasing carbon iber diameters after
3 and 7 days of culture [15]. Compared to larger-diameter carbon
ibers, osteoblasts synthesized more alkaline phosphatase and
deposited more extracellular calcium on nanometer-diameter carbon
ibers after 7, 14, and 21 days of culture [15]. They suggested that
protein-mediated enhanced osteoblast function may be occurring
on the carbon nanoibers.
Figure 10.13
Enhanced osteoblast proliferations on nanophase carbon
iber compacts after 1, 3, and 7 days. Substrates:
borosilicate glass (reference substrate), 100 nm
diameter with a pyrolytic outer core, conventional 200
nm diameter with a pyrolytic outer core, 60 nm diameter
without a pyrolytic outer core, and conventional 125 nm
diameter without a pyrolytic outer core [15].
