Biomedical Engineering Reference
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with many ilopodia extensions from the cell to the substrate
(Fig. 9.34a). In H 2 SO 4 oxidized surface (Fig. 9.34b), the surface area
of cells attachment is minimized. The H 3 PO 4 oxidized surface shows
excellent cell spreading and proliferation (Fig. 9.34c), forming a
three-dimensional ibril network. In the HF treated surface, the
colonization of the cells was noticed, with cells developing a double
net-like layer structure (Fig. 9.34d) [14].
Figure 9.33 SEM images illustrating OPC1 cell adhesion after 3 days of
culture on (a) control-Ti and oxide surfaces anodized in (b)
H 2 SO 4 , (c) H 3 PO 4 and (d) HF electrolytes [14].
Figure 9.34 SEM images showing the cellular adhesion and proliferation
on (a) control Ti, oxide surfaces anodized in (b) H 2 SO 4 , (c)
H 3 PO 4 and (d) HF electrolytes after 11 days of incubation
[14].
 
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