Studying Cell–Surface Interactions In Vitro: A Survey of Experimental Approaches and Techniques - Tissue Engineering: Cell-Surface Interactions for Tissue Culture - page 64

amplitude of the resonator's shear displacement during cell attachment, the authors
were able to determine a threshold lateral oscillation amplitude of *20 nm in the
center of the resonator (driving voltage: [ 5 V) beyond which cell adhesion to
the quartz surface was retarded or even entirely blocked. A maximum shear
amplitude of 35 nm (driving voltage: 10 V) was sufficient to completely inhibit
cell adhesion for all cell lines under study. However, shear oscillations of similar
amplitudes were unable to disrupt cell-surface interactions within established cell
layers. The cells could not be displaced from the surface once they had formed
mature adhesions. In the long run, this method might develop into a new approach
to probe the mechanical shear resistance of cell-surface interactions. As it is
possible to coat the resonator with different biomaterials without losing its sensor
function, the assay can be used to characterize a wide variety of materials.

5 Synopsis

Table 1 summarizes the various approaches and techniques for studying cell-surface
interactions as they have been described in this survey. We have included the
individual strengths and limitations of each technique according to our personal
perspective and judgment. The survey is not exhaustive but focuses on those
assays and concepts that have given or are expected to give significant input to a
better understanding of cell-surface interactions.

Acknowledgments The authors would like to express their gratitude to the Kurt-Eberhard Bode
Stiftung (Germany) for financial funding.

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