Interaction ofCartilage andCeramic Matrix - Tissue Engineering: Cell-Surface Interactions for Tissue Culture - page 310

Table 2 Remaining cells per carrier with different surface structures after removing constructs

(n =4)
Surface
modification

As received Polished using

plain paper

Polished using
polymer sheet

Surface Rough and inhomogeneous Smooth Structured

31.8 9 10 3 6.1 9 10 3 6.4 9 10 3 4.3 9 10 3 5.2 9 10 3 2.4 9 10 3

Cells per carrier ± ± ±

0.05 30

0.04

20

0.03

0.02

10

0.01

0.00 0

rough smooth structured rough structured

smooth

polished polished polished

polished

Fig. 9 Young's modulus and GAG content of cartilage-carrier constructs cultured on rough and
inhomogeneous surfaces of the carriers as received, on smooth surfaces of the carriers polished
with plain paper and on structured surfaces of the carriers polished with polymer sheet (n = 4-6)

Fig. 10 Histological sections stained with Safranin O of cartilage cultured on a rough and
inhomogeneous surfaces of the carriers as received, b smooth surfaces of the carriers polished
with plain paper and c structured surfaces of the carriers polished with polymer sheet

Results are comparable with the formation of the cell layer which is necessary to
trigger connection between carrier and tissue.
Young's modulus and GAG content were also measured as quality parameters.
Constructs using carriers with modified surface structures showed slightly higher
Young's moduli than those using untreated carriers. Differences in the biochemical
parameters could hardly be observed when comparing cartilage-carrier constructs
with modified surface topographies among each other (Fig. 9 ). Comparing histo-
logical sections, glycosaminoglycans are unequally distributed in constructs grown
on the top of untreated carriers (Fig. 10 )[ 60 ].