Biomedical Engineering Reference
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50
40
30
20
10
0
0
2000
4000
t ( s )
6000
8000
(b)
0
500
1000
1500
2000
2500
3000
0
0.5
1
1.5
2
2.5
[L] tot /[M] tot
(c)
0
Affinity constant (M 1 )
0.2
10 4
0.4
0.6
10 5
0.8
10 8
10 6
10 7
1
0
0.5
1
[L] tot /[M] tot
1.5
2
(a) Power dissipation in an ITC experiment after successive injections of 10 μL solutions containing
the ligand into a macromolecular solution; (b) total heat exchanged in terms of the ratio of molar
concentrations of ligand to macromolecule, showing the saturation of the complex formation;
(c) simulation of titration curves of a 10 - 4 M solution for various values of the affinity constant K a .The
vertical axis shows the heat released, normalized by the total heat if all the ligand injected interacts. The
optimum choice of affinity constant for such experiments is on the order of 10 6 M 1 , where the product
c = K a [M] 0 of the affinity constant and the molar concentration of the receptor in the cell is
approximately 100. Adapted with permission from Bruylants and Bartik ( 2005 ).
Figure 2.10
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