Design and Synthesis of Photoreactive Polymers for Biomedical Applications - Biomimetics: Advancing Nanobiomaterials and Tissue Engineering - page 263

• Protein
• Antigens

New technology

N 3

N 3

N 3

OH

NH 2

COOH

COOH

S-S OH

NH 2

Photoirradiation

NH 2

Impossible to immobilize
by the same method

Figure 11.11 Because proteins differ chemically and antigens are heterogeneous,
it is diffi cult to immobilize them all using the same methods (left). The
photoimmobilization method was invented (“New Technology”) to immobilize
various biomolecules covalently, including proteins (right).

Suppression of nonspecific adsorption

• No polymer coating

Antibodies

Nonspecific adsorption

• Bio-nonfouling photoreactive polymer coating

No nonspecific adsorption
high S/N ratio

Figure 11.12 There are advantages in using bio-nonfouling polymers for
preparing microarrays. These polymers can immobilize organic molecules and
inhibit the nonspecifi c adsorption of proteins including antibodies.