Biomedical Engineering Reference
In-Depth Information
O
O
H
HN
N
WSC
+
Gelatin-NH 2
O
N
Room temp/2h
O
N
Gelatin-NH
CO
( a )
COOH
WSC-water-soluble carbodiimide
R 1
O
O
H
+
R 1
COOH
N
N
N
N
.HCl
N
N
.HCl
R 2
(NH 2 ) n
(Gelatin)
( b )
O
O
(NH 2 ) n-m
NH
R 2
R 1
+
N
H
N
N
.HCl
H
m
O
Where R 1 is
Figure 11.7 Synthetic methods for photoreactive gelatins. Modifi cation with
(a) 1-(2-carboxyethyl)thymine and (b) benzophenone.
biodegradability [28]. The scheme for manufacturing benzophenone-
derivatized UV-reactive gelatin is shown in Figure 11.7b.
As well as organic or biological surfaces, immobilization of proteins
on metal surfaces such as titanium and alloys has become important for
medical applications. Mojgan et al. [29] immobilized titanium with pho-
toreactive gelatin carrying azidophenyl groups using silane coupling.
Weng et al. [30] prepared a Ti-O surface engineered with self-assembling
3-aminopropylphosphonic acid (APP), which was further immobilized
with azidophenyl gelatin (azidophenyl gelatin was prepared by reacting
the carboxyl groups in gelatin to react with 4-azidoaniline, as shown in
Figure 11.4b). The APP made an organic bond with the azidophenyl gela-
tin. The micropattern onto the immobilized surface was made using pho-
tomasking against UV irradiation. The micropattern was confi rmed by
staining with Sirius red and the surface profi le was analyzed. The surface
was allowed to grow human endothelial EVC304 cells and the cell attach-
ment and growth showed the pattern produced during UV irradiation
and photomasking. Thus, an azidophenyl gelatin-modifi ed Ti-O surface
can be used for the micropatterning of endothelialization in vitro [30].
Recently, photoreactive azidophenyl groups were also introduced to fi sh
gelatin to create an azidophenyl-fi sh gelatin compound and the curing
 
Search WWH ::




Custom Search