Biomedical Engineering Reference
In-Depth Information
Photoreactive polymers (+ molecules to be immobilized ) in solution
Substrate
Substrate
Dry
Dry
UV irradiation
UV irradiation
Photo-mask
Wash
Wash
( a )
( b )
Figure 11.5 Preparative methods using (a) photolithography and (b) microarrays
using UV-sensitive crosslinkable macromolecules.
Mask
CBB
1
200 μ m
150 μ m
100 μ m
500 μ m
Figure 11.6 Micropattern immobilization of photoreactive human gelatin.
Immobilized proteins were stained with Coomassie Brilliant Blue (CBB).
human mesenchymal stem cells, and its applicability was investigated
by its capacity to bind to various polymers employed in tissue engineer-
ing studies. In addition, its combination with engineered growth factors
with a collagen/gelatin affi nity was shown to exhibit tissue-repairing
activities—such as epidermal cell proliferation, angiogenesis, and endo-
thelialization on and/or around the implanted materials—and was also
investigated.
We have immobilized insulin [12-14], epidermal growth factor (EGF)
[15-20], nerve growth factor [21], erythropoietin [22], leukemia inhibitory
factor [23], and vascular endothelial growth factor [24] using photoreactive
Search WWH ::




Custom Search