Hydroxyapatite-Biodegradable Polymer Nanocomposite Microspheres Toward Injectable Cell Scaffold - Biomimetics: Advancing Nanobiomaterials and Tissue Engineering - page 232

1000

800

600

400

200

0

HAp-coated
PLLA

Bare
PLLA

Figure 9.11 The number of cells adhered on each samples after 24-h incubation.
The error bar represents standard error (n = 30). *P < 0.05. Reprinted with
permission from [52]. Copyright © 2009 American Chemical Society.

sample after trypsin-EDTA treatment using the optical microscope and
the Burker-Turk type cell plate also indicated an effectiveness of the HAp
nanoparticle coating on the PLLA mircospheres toward cell adhesion; the
number of the cells adhered on the HAp nanoparticle-coated microsp-
heres and the bare PLLA microspheres were measured to be 1.5

× 10 5 and

1.0 4). It has also been confi rmed

that the HAp-coated biodegradable microspheres showed an improved
cell adhesion property for bone marrow mononuclear cells (BMNCs)
which are used for cell-based therapeutic angiogenesis (see Section 9.5).
The cell-microsphere adhesive force was measured with a colloidal
probe atomic force microscope, where the nanocomposite microspheres
with PLLA core/HAp shell morphology or bare PLLA microspheres were
used as the probe. According to the evaluation of the measured force
curve, the cell adhesive force increased about 2.8 times thanks to the pre-
sence of HAp on the PLLA microsphere surface [56].

× 10 5 cells/well, respectively (P<0.05; n =

9.5 Application of HAp-Biodegradable Polymer

Nanocomposite Microspheres as an Injectable
Scaffold

It has been confi rmed that the HAp nanocrystal-coated biodegradable
microspheres can work as an effective injectable scaffold, which enhances