Biomedical Engineering Reference
In-Depth Information
Fig. 26.1
Measuring principle, components, and assembly of the aspiration probe. (
a
) The CCD-
camera is non-sterile mounted to the assembled probe through a long sterile bag taped to the rear
probe end and sealed from the aspiration tube via an optical glass. (
b
) The tissue is aspirated
through a 10 mm hole and the resulting deformation side view profile captured as it is reflected in
an optical prism to the camera mounted at the top of the probe
26.2 Methods
This section introduces the experimental methods for intraoperative aspiration mea-
surements, Glisson's capsule characterization, and the corresponding data analy-
sis procedures.
1
The procedure for intra-operative measurements on humans must
be non-traumatic, operate under sterile conditions and comply with the time- and
space-limitations in the operating room, while at the same time allowing to main-
tain control over the mechanical boundary conditions and the protocol applied in
the test.
26.2.1 Aspiration Device
The working principle of the aspiration device is illustrated in Fig.
26.1
. The pro-
cedure is based upon the so called 'pipette aspiration' technique. The instrument
consists of a tube closed on one extremity by a disk containing the circular open-
ing (diameter: 10 mm) for tissue aspiration. The internal pressure in the tube can
1
The work described here constitutes the main contribution of the PhD Thesis of the first author,
see Hollenstein (
2011
). For this reason, text passages, figures, and tables from Hollenstein (
2011
)
are used in this article.
