Biomedical Engineering Reference
In-Depth Information
1) Pre-activation of support
B
Binding
agent
Biomolecule
Support
2) Direct coupling to support
B
or
B
Coupling
via arm
B
3) Pre-activation of biomolecule
B
B
B
B
B
Comonomer
a) Soluble polymer
4)Conjugation followed by
copolymerization
B
B
B
B
B
Activated
monomer
Monomer
conjugated
biomolecule
b) Gel
Surface
radicals
B
B
B
c) Graft copolymer
5) Direct attachment
to pre-activated polymer,
gel or graft copolymer
Same as 4a
above
B
Soluble polymer
Comonomer
Same as 4b
above
Activated
monomer
Crosslinker
B
Gel
Surface
radicals
Same as 4c
above
B
Graft copolymer
Note : B may be immobilized with or without a "tether" arm in any of the above
Fig. 3.2.16-1 Schematic cartoons showing various methods for covalent biomolecule immobilization.
