INTRODUCTION TO FLOW CYTOMETRY - Flow Cytometry in Drug Discovery and Development - page 16

left (Figure 1.9a-d) and analysis of the cells following sorting is shown on the right
(Figure 1.9a 0 -d 0 ). The live cell gate (Figure 1.9a and a 0 ) indicates that following sorting
the cells that were gated on CD4 and the live gate resulted in 99% of the gated cells
meeting the gating criteria (live, singlets, and CD4 þ ).
1.10 CONCLUSION
Since their introduction in the 1970s, the design and applications of flow cytometers
have undergone tremendous change. Current flow cytometers are rapid, use multiple
lasers (five lasers on several instruments), and can detect more than 20 different
fluorochrome tags. Some of the novel applications include fluorescence in situ
hybridization (FISH) using flow cytometer and Amnis ImageStream, which is a
blend of flow cytometry and microscopy and allows the visualization of single cells.
The field of flow cytometry holds great promise for research and clinical diagnostics.
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