INTRODUCTION TO FLOW CYTOMETRY - Flow Cytometry in Drug Discovery and Development - page 14

FIGURE 1.8 Dot plot versus contour plot of the same data. Cells were stained with CD3-FITC, CD16 and CD56-PE, CD4-APC, and CD8-PerCP-
Cy5.5. Plots (b)-(d) and (b 0 )-(d 0 ) show similar results by both dot plot and contour plot. However, the rare population of double positive cells can be
visualized in the dot plot (d) but not in the contour plot (d 0 ). For details refer to the text.