Biomedical Engineering Reference
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FIGURE 1.5
Compensation controls (a-c) human PBMCs stained with a single FITC-labeled antibody. (a) Cells are gated on the lymphoid gate based
on forward scatter (x-axis) and low side scatter (y-axis). (b) Uncompensated data demonstrating that the FITC signal is “spilling” into the PE channel with
65.4% of the cells demonstrating dual positivity incorrectly. (c) Data after compensation with only single positive cells expressing FITC and no PE. Note
that the PE mean for the FITC negative and FITC positive cells is nearly identical. (a
0
-c
0
) Two-color data of human PBMCs stained with anti-CD19
labeled with PerCP-Cy5.5 (B-cell marker) and anti-CD3 labeled with APC (T-cell marker). There should be no dual positive cells. Data were collected as
listmode and compensation was added after data collection: (a
0
) lymphoid gate; (b
0
) uncompensated data; (c
0
) data after compensation. (See the color
version of the figure in the Color Plates section.)
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