Biomedical Engineering Reference
In-Depth Information
(a)
Preconfluent
Confluent
Postconfluent
6000
5000
4000
3000
2000
1000
0
0
25
50
75
100
125
nM labeled drug
(b)
100
Preconfluent
Confluent
Postconfluent
75
50
25
0
0
25
50
75
100
125
Control dilution
(c)
100
P6
P9
90
80
P12
70
P15
P18
60
P20
P23
P26
P29
50
40
30
20
P32
P35
P38
10
0
High PC
Low PC
FIGURE 10.3
Effect of cell growth and culture conditions on assay readouts. The relation-
ship is shown between cell confluency on signal output, GMFI (a) and percent inhibition of
uptake (b). High and low positive controls (PC) are used with different cell culture passage
numbers (passage 6 through 38), the association with percent uptake of fluorescently labeled
biotherapeutic is shown (c).
other related parameters are recorded and tracked along with the assay readout.
When passage-related effects are suspected or identified, larger cell banks can be
made to minimize such effects. Creating master and working cell banks ensures
the investigator that a well-defined and consistent population of cells is available for
use in each assay.
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