Biomedical Engineering Reference
In-Depth Information
Table 6-2. Sources of Variability in Spotting.
Binding of cDNA to Microarray
cDNA Volume Deposited
Digitization of Spot Intensities
Environmental Conditions
Experimental Design
Hybridization of RNA to DNA
Instrument Error
Locating Spotted Areas
Microarray Surface Chemistry
Quality of Spotted Genes on Array
Reagent Preparation
Spot Placement (Robot Arm Accuracy)
The expression matrix format is a more human-readable form than a reproduction of arbitrarily
arranged microarray data. A color version of an expression matrix is more useful in publications and
for quick visual inspection of experimental results than is a table of relative red and green
