Hardware Reference
In-Depth Information
Fig. 2.11
Synthesis results for the bioassay when we use the CCD camera-based sensing system.
(
a
) t D 0: Mix 2 and Mix 3 begin; t D 2: DNA fouling occurs, Mix 3 stops and will be re-executed;
corresponding electrodes are discarded; (
b
) t
D
2: recovery operation Re-Mix 3 begins; t
D
6:
Mix 2 is completed; (
c
) t
D
6: Mix 1 begins and Re-Mix 3 is still being executed; t
D
12:
bothMix1andRe-Mix3arecompleted;(
d
) t
D 12:Mix4begins;t
D 15: Mix 4 is completed;
(
e
) t
D
15: Mix 5 begins; t
D
18: Mix 5 is completed. The whole bioassay is completed at time 18
the erroneous operation. While in the CCD camera-based sensing system, recovery
can be triggered immediately after an error occurs. On the other hand, in the CCD
camera-based sensing system, recovery can only be triggered immediately after an
error occurs.
It is important to note that, light from the camera may influence some biochemi-
cal substances, e.g., fluorescent markers in the droplet [
21
]. Thus in order to monitor
experiments that include photosensitive samples/reagents, we need to choose the
detector-based sensing scheme.
2.4
Error Recovery and Dynamic Re-synthesis
With the availability of hardware that can send feedback to the control software,
it is now necessary to design a physical-aware software that can analyze sensor
data and dynamically adjust the synthesis result. Adaptations include updates for
the schedule of fluid-handling operations, resource binding, module placement, and
droplet routing pathways.
The re-synthesis procedure includes two phases: the first phase is off-line data
preparation before the execution of bioassay and the second phase is on-line
monitoring for the fluid-handling operations as well as dynamic re-synthesis of the
bioassay. Details are presented below.
