Hardware Reference
In-Depth Information
Tabl e 6. 7
Execution time of
bioassays on Chips E
Biochip
G
Chip E
Chip F
Chip G
Bioassay
Multiplexed bioassay
87 s
131 s
NA
PCR bioassay
NA
20 s
NA
Protein bioassay
375 s
566 s
161 s
b
43 5 24 35 9 34 23 21 16 9
16 32 42 40 38 18 30 6 11 27
30 36 13 20 29 31 12 17 24 25
4 41 39 33 8 28 26 19 20 18
40 28 34 16 14 25 21
a
20
34
31928245678
32 26 19 14 4 9 10 11
22 23 6 21 12 13 2 14
4 16 29 25 15 8 16 17
27 20 11 26 1 18 19 3
28 30 12 24 10 20 5 13
25 7 182717212211
3
15 23
2
1
2
3
4
37
3
22 26
7
13
5
1
6
7
39 35 32 29 27 19
4
8
9
10
27 31 21 10 15 22 11 12 13
2
2
36 33 24
1
23 14
3
15 16
23 37
7
30 28 20 10
5
17 18
Fig. 6.19
(
a
) pin-assignment configuration for an 8
8 electrode array; (
b
) pin-assignment
configuration for a 10
10 electrode array
when the bioassay-specific designs (Chips A
D [
9
]andChipsE
G [
18
]) and
general purpose designs (Chips 1
4) are compared.
6.7.3
Simulation Results on Regular Array
Next we simulate bioassays on an 8
8 and a 10
10 electrode array to evaluate
the performance of the proposed general-purpose biochip. The pin-assignment
configurations of the 8
8 and 10
10 biochip are shown in Fig.
6.19
aand
6.19
b,
respectively. There are 32 pins assigned to the 8
8 layout, and 43 pins assigned to
the 10
10 layout. The numbers of control pins for the 8
8 direct-addressing and
cross-referencing biochips are 64 and 16; the numbers of control pins for the 10
10
direct-addressing and cross-referencing biochips are 100 and 20, respectively.
The completion times of executing bioassays on the direct-addressing biochips,
the cross-referencing biochips, and the proposed general-purpose biochips can be
found in Table
6.8
. Compared to the direct-addressing biochips, the general-purpose
biochips can reduce the number of control pins by 50% or more with only a slight
increase in the bioassay completion times.
Tab le
6.8
shows that for the protein dilution bioassay, the execution time on the
8
8 cross-referencing biochip is 20 % longer than that for the proposed 8
8 pin-
limited biochip; for multiplexed bioassay and PCR bioassay, the execution times on
these two biochips are almost the same. For the 10
10 array shown in Table
6.8
,
the cross-referencing biochip leads to lower completion time for the protein assay.
