Chemistry Reference
In-Depth Information
3250
2
1
0
1
Ni(II)/P
Figure 2.9
The dependence of UDS of polynucleotide poly(G-C).poly(G-C) on Ni
2+
concentration in water (1) and in 25% ethanol solution (2)
7
6
4
1
2
3
5
4
−
8
40
000
32
000
∼
ν
, cm
−
1
Figure 2.10
CD spectra of polynucleotide poly(G-C).poly(G-C) in 25% ethanol solution at
different concentrations of Ni
2+
: (1) without Ni
2+
, (2) 0.1 Ni/P, (3) 0.2 Ni/P, (4) 0.25 Ni/P,
(5) 0.3 Ni/P, (6) 0.35 Ni/P, (7) 0.4 Ni/P
Z conformation. It was also demonstrated in our experiments that the Ni
2+
- poly(dG -
dC) ·poly(dG-dC) complex forms the C conformation with wrong base pairs during
the B
Z transition process.
61- 63
Thus, we have demonstrated that UV spectroscopy
is a sensitive method for monitoring conformational transitions in double-helical
D N A .
→
2.3 Model and Mechanisms of Metal -Induced Formation of Point Defects
2.3.1 Double Proton Transfer ( DPT ) in DNA : UV Spectroscopic Measurements
The infl uence of pH on UV spectra of guanine and cytosine, as well as on keto- enol
and amino-imino transformations in guanine and cytosine, has been known for a
