Chemistry Reference
In-Depth Information
Table 13.1
Kinetic parameters for some of the most active artifi cial metallonucleases
Hydrolytic Agent
a
k
cat
(s
−
1
)
K
a
(M
−
1
)
k
ψ
(s
−
1
)
b
k
ψ
(s
−
1
)
c
pH
[bp]
d
(
m
M)
reference
′
′′
Eu(III)
7.0·10
−
5
2.6·10
4
8.0·10
−
6
4.1·10
−
6
7.0
19
39
Er(III)
1.0·10
−
4
1.7·10
4
7.8·10
−
6
3.8·10
−
6
7.0
19
11
Zn(II)-pseudopeptide (50 °C)
-
-
∼
4·10
−
5
(3
m
M)
-
7.0
12
20
Zn-quercetin
-
-
1.7·10
−
4
(100
µ
M)
-
7.2
40
21a
1.3·10
4
Cu(II)-TACH (35 °C)
1.2·10
−
3
7.3·10
−
5
1.3·10
−
5
8.1
7
23
3.0·10
3
Pr(III)
2
-azacrown
2.8·10
−
4
4.1·10
−
6
2.0·10
−
6
7.0
19
27
1.0·10
3
Er(III)
2
-shiff macrocycle
1.0·10
−
3
5.0·10
−
6
-
7.0
-
28
Ce(IV)
2
-HXTA
-
-
1.4·10
−
4
(10
m
M)
1.4·10
−
4
(r = 0.07)
8.0
150
29
Fe(III)
2
-DTPB
-
-
2.1·10
−
3
(100
m
M)
-
7.0
77
33
Zn(II)
2
-peptide
-
-
1.0·10
−
5
(3.6
m
M)
1.0·10
−
5
(r = 0.3)
7.0
12
35
5.5·10
3
Co(III)-cyclenC
6
ammonium
2.7·10
−
4
7.2·10
−
6
3.5·10
−
6
7.0
19
43
Zn(II)-bipy-ammonium
-
-
3.8·10
−
5
(50
m
M)
-
38
44
2.5·10
5
Cu(II)-neamine
5.2·10
−
4
2.8·10
−
4
3.1·10
−
4
7.3
50
25
Eu(III)-naphtocrown
1.7·10
3
7.0
19
39
5.8·10
−
4
4.9·10
−
6
2.3·10
−
6
Zn(II)-Rh-peptide
-
-
6.0
40
38
2.5·10
−
5
(5.0
m
M)
2.5·10
−
5
Zn(II)-TACH-anthraquinone
-
1.0·10
4
7.0
12
41
4.6·10
−
6
(5.0
m
M)
1.9·10
−
6
a
Reactivity at 37 °C unless otherwise stated.
b
Pseudo fi rst-order rate constant for the cleavage of plasmid DNA at a fi xed concentration of metal complex (5
M).
c
Pseudo-fi rst-order rate constant for the cleavage of plasmid DNA at a fi xed metal complex to DNA (base pair) ratio (0.125), unless otherwise stated.
d
Concentration of plasmid DNA reported as base pairs.
µ
