Chemistry Reference
In-Depth Information
Table 12.2
Nuclease activity of transition metal complexes-peptide conjugates on super-coiled plasmid
Sequence selectivity
†
Mechanism
of cleavage
% nicked
plasmid DNA
e
% linear
plasmid DNA
e
Reference
[Ru(bpy)
2
(m-GHK)]
2+
oxidative
65 (10)
-
Mainly G
*
24
[Rh(phi)
2
(bpy
′
)]-P1
a
-
40 (18)
4 (0)
Modest selectivity
for 5
54
′
-Pu-Py-Pu-3
′
*
hydrolytic
44 (18)
38 (0)
54
[Rh(phi)
2
(bpy
′
)]-P1 + Zn(II) 1:1
5
′
-Py-Py-Pu-Pu-Pu-3
′
oxidative
45 (18)
-
54
[Rh(phi)
2
(bpy
′
)]-P1 + Cu(II) 1:1
5
′
-Py-Py-Pu-Pu-Pu-3
′
hydrolytic
50 (18)
-
nd
54
[Rh(phi)
2
(bpy
′
)]-P1 + Cd(II) 1:1
hydrolytic
20 (18)
-
nd
54
[Rh(phi)
2
(bpy
′
)]-P1 + Fe(II) 1:1
-
38 (26)
-
nd
54
[Rh(phi)
2
(bpy
′
)]-Bam
b
hydrolytic
54 (26)
2 (0)
nd
54
[Rh(phi)
2
(bpy
′
)]-Bam + 100 eq Zn(II)
hydrolytic
57 (26)
4 (0)
nd
54
[Rh(phi)
2
(bpy
′
)]-Bam + 100 eq Cd(II)
hydrolytic
41 (26)
-
nd
54
[Rh(phi)
2
(bpy
′
)]-Bam + 100 eq Mg(II)
oxidative
62 (26)
3 (0)
nd
54
[Rh(phi)
2
(bpy
′
)]-Bam + 1 eq Cu(II)
oxidative
35 (26)
2 (0)
nd
54
[Rh(phi)
2
(bpy
′
)]-Bam + 1 eq Fe(II)
)]-
a
A
c
oxidative
-
-
*
48
[Rh(phi)
2
(phen
′
5
′
-NACAA-3
′
)]-PA
d
oxidative
-
-
*
46
[Rh(phi)
2
(phen
′
5
′
-CCA-3
′
† Cleavage sites are underlined.
* Samples have been irradiated.
nd
Sequence selectivity has not been determined.
a
P1 is a
de novo
designed peptide incorporating histidine residues for Zn
2+
coordination.
b
Bam is a peptide derived from the active center of BamHI.
c
a
A
is a peptide derived from the recognition helix of phage 434 repressor protein.
d
PA is a peptide derived from the
a
3
helix of the phage P
22
repressor.
e
The control experiment (plasmid only) is given in parenthesis.
