Chemistry Reference
In-Depth Information
Figure 9.3
Crosslinking promoted by annealing. The reactive species is either a vinyl group
(A) or a quinone-methide moiety (B)
Although experiments leading to platinated oligonucleotides have often been
followed by crosslinking studies with no clear-cut separation between the two
aspects of the study, in this chapter the description of these studies has been split
into three sections. Section 9.3 covers most of the methods developed to prepare
platinated oligonucleotides, either using preplatinated monomers that are incorpo-
rated into oligonucleotide chains, or platinating fully assembled oligonucleotides
(partially protected or unprotected, unmodifi ed or containing modifi ed nucleo-
bases). Then, the use of platinated oligonucleotides in studies whose fi nal goal is the
formation of crosslinked duplexes, most often to achieve improved antisense effects,
is described in Section 9.4. Finally, Section 9.5 reviews the results of experiments
aiming at the formation of complexes between platinated oligonucleotides and
dsDNA, namely the formation of triple helices (antigene approach).
Although the purpose of that study was not to establish a crosslink, it is worth
mentioning that the effect of platinating the sense strand of an siRNA duplex was
also evaluated in a recent publication.
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