Biomedical Engineering Reference
In-Depth Information
(A)
(B)
Detaching cell
sheet
Confluent cells on TRCS
(D)
(C)
Cell sheet
(E)
TRCS
(E)
(F)
Detached cell sheet
Figure 5b.1
A cell sheet detaching itself from a temperature-responsive
culture surface (TRCS) by simply reducing culture temperature.
Confluent cells on the TRCS (A) are detached as an intact cell
sheet (B, C, D, E) without cell residues by decreasing the culture
temperature to 20°C. (F) A cell sheet detached from the TRCS.
The dish is a 100 mm culture dish.
infarction model [25]. It is suggested that angiogenesis-related
cytokines (vascular endothelial growth factor [VEGF], hepatocyte
growth factor [HGF]) and a chemokine (stromal cell-derived
factor-1 [SDF-1]) produced from transplanted SkM sheet patches
are partly responsible for the therapeutic effects. While VEGF and
HGF lead to neovascularization, HGF attenuates negative cardiac
tissue remodeling, including cell apoptosis and tissue fibrosis in an
