Biomedical Engineering Reference
In-Depth Information
with the Food and Drug Administration Good Laboratory Practice
Regulations [9]. Recently, the regenerative medicine company
Theregen Inc. reported clinical studies using 3DFC, renamed
Anginera, for the treatment of heart disease [10]. In 2006 a phase I
safety study was initiated of Anginera used as an adjunct therapy in
patients with reversible myocardial ischemia undergoing coronary
artery bypass graft (CABG) surgery. In 2009, a phase I study in
patients who received a left ventricular-assist device (LVAD) as
a bridge to heart transplantation was conducted. Theregen Inc.
concluded that Anginera has the potential for growing mature blood
vessels in underperfused cardiac tissue, which improves perfusion,
and thus stimulates heart tissue repair.
5a.3.3 Poly(Glycolide-co-Caprolactone)
PGCL is a copolymer synthesized from glycolide and ε-caprolactone,
possessing properties of rubber-like elasticity and biodegradability.
Piao et al. implanted rat bone marrow-derived mononuclear cell
(BMMNC)-seeded porous PGCL scaffolds onto the epicardial surface
in a rat MI model [11]. As a result, they observed BMMNC migration
into the epicardial region, differentiation to cardiomyocytes,
induction of neovascularization, and the improvement of lessened
LV remodeling and progressive LV systolic dysfunction.
5a.3.4 Poly(Lactide-co-Caprolactone)
PLCL is a synthetic biocompatible copolymer of L-lactide and
ε-caprolactone, which has elastic and biodegradable characteristics.
Jin et al. investigated the effect of rat bone marrow-derived MSC
implantation with porous PLCL scaffolds in a rat acute MI model
[12]. MSC-seeded PLCL patches attenuated LV dilation and improved
cardiac function. Moreover, implanted MSCs survived in the injured
myocardium and expressed cardiac markers such as MHC, α-actin,
troponin-I, and GATA-4. Additionally, Ozawa et al. showed that
rat aortic SMCs could be seeded onto a PLCL sponge reinforced
with knitted poly-L-lactide fabric. The SMC-seeded patches were
then employed to replace a surgically created defect in the right
ventricular outflow tract (RVOT) of rats [13, 14]. At right weeks after
implantation, degradation of the patches, the presence of elastin
fibers, and endothelialization were all observed in the grafts. Even
