Biomedical Engineering Reference
In-Depth Information
Chapter 4b
Cell Sheet-Based Tissue Engineering
Yuji Haraguchi, Noriko Yasuda, Tatsuya Shimizu, Masayuki Yamato,
and Teruo Okano
Institute of Advanced Biomedical Engineering and Science,
Tokyo Women's Medical University, 8-1 Kawada-cho,
Shinjuku-ku, Tokyo, 162-8666, Japan
yharaguchi@abmes.twmu.ac.jp
4b.1 Introduction
Cell-based therapy using the direct injection of dissociated cells
has been progressing rapidly, and some clinical trials have already
started [1-3]. On the other hand, cell injection therapy has several
problems, which remain to be solved, the most severe of which is
the significant cell loss and death after transplantation [4, 5]. Tissue
engineering has become the second generation of cell therapy, with
the potential to overcome these problems [1]. Tissue engineering
is based on the concept that three-dimensional (3D) biodegradable
scaffolds can be used as an alternative to the extracellular matrix
(ECM) into which living cells can be seeded [6]. Engineered tissues
can be cultured in vitro and then transplanted into various tissues
