Biology Reference
In-Depth Information
Table 10.1
(continued)
Taxonomic
algal group
Algal species
(sampling location)
Major bacterial phyla
encountered
Representative
species
Number of
clones/
sequences
analyzed
Methods
Experimental details
References
Halimeda opuntia
(coral reef, Curacao,
Netherlands Antilles)
Proteobacteria (31-34%)
Cyanobacteria (27%)
Unknown bacteria
(10-12%)
Bacteroidetes (15%)
Firmicutes (6-8%)
Cyanobacteria
Group I (8%)
52,100,
44,800
c
PYR
Direct tissue lysis
Comparison of four algae and
reef-building coral spp.
Barott et al. (
2011
)
Functional groups
Turf algae
(coral reef, Curacao,
Netherlands Antilles)
Proteobacteria (49-57%)
Unknown bacteria (10%)
Cyanobacteria (5-11%)
Firmicutes (8-9%)
Bacteroidetes (5-13%)
Actinobacteria (2-3%)
Acidovorax
spp.
(11%)
34,000
61,000
c
PYR
Direct tissue lysis
Comparison of four algae and
reef-building coral spp.
Barott et al. (
2011
)
Crustose coralline algae
(coral reef, Curacao,
Netherlands Antilles)
Proteobacteria (27-32%)
Unknown bacteria (25%)
Cyanobacteria (12-20%)
Firmicutes (10-18%)
Bacteroidetes (15%)
Chloroflexi (7%)
Unclassified
Bacteria
(11%)
39,600
36,600
c
PYR
Direct tissue lysis
Comparison of four algae and
reef-building coral spp.
Barott et al. (
2011
)
TRFLP
, terminal restriction fragment polymorphism analysis;
DGGE
, denaturing gradient gel electrophoresis;
CU
, culturing;
CL
, clone libraries;
CL-RFLP
, representative clones sequenced after restriction
fragment length typing of clones;
Bdseq
, DGGE band sequencing;
SP
, specific primers;
cFISH
, catalyzed reporter deposition fluorescence hybridization; PYR, pyrosequencing; n.d., not determined
a
~60 Sequences per alga and season analyzed, DNA pooled from three individuals
b
Clones (
n
170) were from the Baltic Sea algal sample, and 112 clones were from the North Sea algal sample; however, a large number of sequences was from chloroplast 16S rRNA genes (
n
228)
¼
¼
c
Each algal species was analyzed from two different coral sites
d
~50 clones per host analyzed by RFLP
e
Clones from six libraries representing six different
Ulva australis
samples; in addition, ten different seawater samples were analyzed
