Biology Reference
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Table 10.1 16S rRNA gene sequence-based community composition of microorganisms associated with macroalgae
Taxonomic
algal group
Algal species
(sampling location)
Major bacterial phyla
encountered
Representative
species
Number of
clones/
sequences
analyzed
Methods
Experimental details
References
Rhodophyta
(Red algae)
Delisea pulchra (Bare
Island, Botany Bay,
Sydney, Australia)
a -, d - and
g -Proteobacteria,
Planctomycetes,
Bacteroidetes
n.d.
236/62
CL-RFLP
DGGE
Direct tissue lysis
Comparison of three host
species (sponge, red, and
green macroalgae)
Longford et al. ( 2007 )
Ahnfeltiopsis
flabelliformis
Caulacanthus ustulatus
n.d
-/23
DGGE
Bandseq
SP
Direct tissue lysis
Tracing cyanobacteria on
macroalgal surfaces
Ohkubo et al. ( 2006 )
Cyanobacterium
Acaryochloris spp.
Porphyra yezoensis
(cultivated in the lab)
g
-Proteobacteria,
b
Alteromonas spp.
100/100
CL
Direct tissue lysis
Development of a primer for
excluding eukaryal templates
Namba et al. ( 2010 )
-Proteobacteria,
Bacteroidetes
60/60 a
Gracilaria
vermiculophylla (Kiel
fjord, North Sea)
a
-Proteobacteria (31-71%)
Bacteroidetes (9-41%)
Actinobacteria (3-11%)
d
Mesorhizobium spp.
(~25%)
DGGE
CL,
Cotton swab samples
Comparison of three algae
and seawater; summer and
winter samples
Lachnit et al. ( 2011 )
-Proteobacteria (~10%)
Planctomycetes (~3%)
170 b
112 b
Phaeophyta
(Brown algae)
Laminaria saccharina
(Kiel, Baltic Sea;
Helgoland, North Sea)
a
-Proteobacteria
(9 phylotypes),
g
Erythrobacter spp.
Roseobacter spp.
DGGE
CL
Specific microbial
populations associated with
different parts of the alga,
some overlap with bacteria in
seawater found
Staufenberger et al.
( 2008 )
-Proteobacteria (6)
Bacteroidetes (4)
Laminaria hyperborea
(Bergen, Norway)
Planctomycetes
RB1 lineage
(73-98%)
260
CU
SP
CL
(FISH)
scraped biofilm
Over 50% of epiphytic
microbial cells (DAPI) were
Planctomycetes, seasonal
variability
Bengtsson and Ovreas
( 2010 )
Laminaria hyperborea
(Bergen, Norway)
Planctomycetes (47%)
a
n.d.
273 bands
DGGE
Bdseq, scraped biofilm
Seasonal variation of
microbial communities and
comparison with seawater
Bengtsson et al. ( 2010 )
-Proteobacteria (21%)
Verrucomicrobia (18%)
Cyanobacteria (5%)
Bacteroidetes (4%)
g -Proteobacteria (4%)
Laminaria hyperborea
a -Proteobacteria
Roseobacter spp.
Follow-up study, proves that
cultivation could not retrieve
the most relevant bacteria
from the natural biofilm
Bengtsson et al. ( 2011 )
 
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