Biomedical Engineering Reference
In-Depth Information
No scaffold/Inactive scaffold
Active scaffold
MFB
MFB
MFB
MFB
Fig. 9.1
Immunohistochemical localization of αSMA in the contractile cell capsule in skin
wounds (
top row
,
red
, 10 days post injury) and peripheral nerve wounds (
bottom row
,
brown
, gap
midpoint, 7 days post injury).
Left column
: control wounds (
top left
: ungrafted skin;
bottom left
:
nerve grafted with silicone tube).
Right column
: wounds grafted with active collagen scaffolds.
Arrows
: scaffold struts.
Scale bars
: 100 µm.
MFB
myofibroblasts
F
Fig. 9.2
Fibroblasts inside two collagen scaffolds at the center of a skin defect in the guinea pig,
day 7.
Left
: View of highly dense, clustered cells inside the large pores of an inactive scaffold
with pore size about 400 µm, specific surface ca. 2000 mm
2
/mm
3
Arrows: scaffold strats.
Right
:
Isolated cells at very low density inside the small pores of an active scaffold, pore size about
40 µm, specific surface ca. 25,000 mm
2
/mm
3
.
F
fibroblasts
1989), while analog B did not delay contraction significantly and was considered
inactive (Fig.
9.3
). The histologic evidence shows that the cell density inside the
pores of inactive analog B was much higher than in the pores of active DRT scaffold
