Biomedical Engineering Reference
In-Depth Information
array-partitioning-based method described in Section 3.1 leads to a comple-
tion time of 73 s. However, it requires 35 control pins, that is, an increase of
40% compare to the broadcast-addressing method.
3.3.4.2 Polymerase Chain Reaction (PCR)
For the second assay, we use the mixing stages of the PCR. These stages are
used for rapid enzymatic amplification of specific DNA strands. Recently,
the feasibility of performing droplet-based PCR on digital microfluidics-
based biochips has been successfully demonstrated [26]. Its assay protocol
can be modeled by a sequencing graph, as shown in Figure 3.37. Mapping
the protocol on to the array, we obtain the chip layout and schedule shown
in Figures 3.38 and 3.39, respectively.
Tris-HCL
(pH 8.3)
Bovine serum
albumin
Beosynucleotide
triphosphate
AmpliTa g
DNA
KCL
Gelatin
Primer
Lamda DNA
Mix
Mix
Mix
Mix
Mix
Mix
Mix
Figure 3.37
Sequencing graph for the mixing stage of PCR.
KCL
Tris-HCL
(pH 8.3)
Lamda DNA
Bovine
serum
albumin
AmpliTag
DNA
Beosynucleotide
triphosphate
Gelatin
Primer
Figure 3.38
Mapping of the PCR assay on a 15 × 15 array.
