Biomedical Engineering Reference
In-Depth Information
Nop
S
2
S
2
R
1
R
2
R
1
R
2
S
1
S
1
I
5
I
6
I
7
I
8
I
1
I
2
I
3
I
4
M
1
M
2
M
3
M
4
D
1
D
2
D
3
D
4
Figure 3.34
Sequencing graph model for the multiplexed bioassay.
S
1
and
S
2
are samples,
R
1
and
R
2
are
reagents,
M
1
~
M
4
are mixing operations, and
D
1
~
D
4
are detection operations.
In the multiplexed assay, eight droplets (two droplets from each sample/
reagent) are dispensed and routed to the mixer located at the center. Next,
four mixing and detection operations are carried out in a pipeline manner
following the schedule shown in Table 3.2. We assume that the droplets are
transported at the rate of 1 electrode/second (i.e., 1 Hz).
Next, we apply the proposed broadcast-addressing method to the preced-
ing chip layout. As shown in Figure 3.11, the multiplexed-assay chip utilizes
59 electrodes. We calculate the electrode-activation sequences based on the
scheduling and routing result presented in Subsection 3.1.4. A fragment of
the activation sequences is listed in Table 3.6. Next, the clique-partitioning-
based broadcast-addressing method is used to generate the electrode con-
nections and the pin-assignment plan. The results are shown in Figure 3.35.
The pins assigned to the electrodes are shown in the corresponding boxes.
TAble 3.6
A Fragment of the Activation Sequences for Multiplexed Assay
Electrode # (7~20)
Activation Sequences (0s~13s)
…
…
…
…
…
…
…
…
…
…
…
…
…
…
7
1
0
x
x
x
0
1
0
x
x
x
0
…
8
0
1
0
x
x
x
0
1
0
x
x
x
…
9
x
0
1
0
x
x
x
0
1
0
x
x
…
10
x
x
x
x
x
x
x
x
x
x
x
x
…
12
x
x
x
x
x
x
x
x
x
x
x
x
…
13
x
0
1
0
x
x
x
0
1
0
x
x
…
14
0
1
0
x
x
x
0
1
0
x
x
x
…
15
1
0
x
x
x
0
1
0
x
x
x
0
…
16
x
x
0
1
0
x
x
x
0
1
0
x
…
…
…
…
…
…
…
…
…
…
…
…
…
…
…
