Biomedical Engineering Reference
In-Depth Information
Te st Operations
Functional Operations
E
1
E
2
E
3
E
4
E
1
E
2
E
3
E
4
E
1
E
2
E
3
E
4
T
1
E
1
Electrode
E
2
E
3
E
4
E
1
E
2
E
3
E
4
Activation
X
X0 1
Sequence
X
01 0
0100
100X
T
2
Electrode
E
1
E
2
E
3
E
4
Activation
X
X01
T
3
Electrode
E
1
E
2
E
3
E
4
Sequence
X
010
0101
Activation
Sequence
XX 01
X 010
0 100
100X
XX 01
X
Functional droplet
Te st droplet
010
0
101
Figure 5.3
Illustration of the influence by adding test operations to the bioassay.
fluidic operations with the droplet manipulation steps needed for the target
bioassay. The merging can be carried out by attaching the electrode-activation
sequences for the test procedure to the electrode-activation sequences for
the target bioassays. For each electrode in the array, its activation sequence
during the test procedure is added to that for the target bioassay to form a
longer sequence. If these longer electrode-activation sequences are provided
as input to the broadcast-addressing method, the resulted chip design will
support not only the target bioassay but also the test operations.
We use an example to illustrate the details of the preceding DFT method.
Figure 5.3 shows a linear array consisting of four electrodes. A simple
“routing assay” is mapped to the array, where a droplet is to be routed from
E
4
to E
1
, one electrode per step. We first list the activation sequence for each
electrode-activation sequences for the splitting test are shown in table T
2
of
Figure 5.3. These activation sequences are then combined with the activation
sequences in table T
1
. The resulting longer activation sequences are listed
