Lipid-Based Pharmaceutical Nanocarriers for Imaging Applications - Nanotechnology for Biomedical Imaging and Diagnostics - page 54

NH-CBZ

NH-CBZ NH-CBZ

+

NH-CBZ NH-CBZ NH-CBZ NH-CBZ

HOOC

CBZ-protected poly- L -lysinelysine NGPE

Carbonyldiimidazole
N -hydroxysuccinimide

NH-CBZ NH-CBZ NH-CBZ

HN-OC

NH-CBZ NH-CBZ

NH-CBZ NH-CBZ

N , α-(ε-CBZ-polylysyl)-NGPE

HBr/acetic acid

NH 2 NH 2 NH 2

HN-OC

NH 2 NH 2 NH 2 NH 2

Deprotected N , α-(ε-CBZ-polylysyl)-NGPE

DTPA cyclic anhydride

DTPA DTPA DTPA

NH NH NH

HN-OC

NH
DTPA

NH
DTPA

NH
DTPA

NH
DTPA

N , α-(DTPA-polylysyl)-NGPE

100.0

80.0

60.0

40.0

20.0

0.0

DTPA-PE PAPs
figure 3.2 synthesis of amphiphilic polychelate dTpA-pLL-NgpE consisting of hydro-
philic dTpA-polylysyl (pLL) (dTpA-pLL) moiety and hydrophobic N-glutaryl phosphatidyl-
ethanolamine (NgpE) moiety ( top ); labeling efficiency of liposomes containing the same
molar fraction of pAp or dTpA-pE ( bottom ).