Future Potential Clinical Applications of Spectroscopic Techniques in Human Diseases - Vibrational Spectroscopy for Tissue Analysis - page 307

0.9 Black : Grade I

Red : Grade II
Blue : Grade III

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

3000 2500 2000

Raman Shift (cm -1 )

1500 1000 500

Figure 5.2
Raman spectra of different IDC grades.

0.75
0.70
0.65
0.60
0.55
0.50

Black : LNG
Blue : ING
Red : HNG

0.45
0.40
0.35
0.30
0.25
0.20
0.15
0.10

3000 2500 2000 1500 1000 500

Raman Shift (cm -1 )

Figure 5.3
Raman spectra of different DCIS grades.

1.5

1442
1662

1260

2930
2959

2880 1955

2851

1.0

540

780
850
992

1096

0.5

650

0.0

3000 2500 2000 1500 1000 500

Wavenumber/cm -1

Figure 5.4
Raman spectra of normal (red), DCIS (HNG) (blue), and IDC (GIII) (black) breast tissues. Bottom
traces are differences between IDC (black) and control, and DCIS (blue) and control tissues. For
calculating the differences, spectra have been normalised to the peak at 1093 cm -1 . Horizontal
double lines indicate the level of noise in order to facilitate justification of peak significance.