FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 272

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

ν as (COO − ) (polysaccharides,
pectin)

* 7

1606 cm −1 Adenine vibration in DNA * 23

1607 cm −1 Tyrosine, phenylalanine ring

vibration

* 43

1608/9 cm −1 Cytosine (NH 2 ) * 21

1609 cm −1 Adenine vibration in DNA * 23

Haemoglobin * 60

1610 cm −1 Cytosine (NH 2 ) * 21

C= C bending in phenylalanine
and tyrosine

* 16

1613 cm −1 Ring stretch with CO conjugation * 14

1614 cm −1 Tyrosine * 3

1615 cm −1 Tyrosine, Tryptophan, C= C

(protein)

* 12

1616 cm −1 C= C stretching mode of tyrosine

and tryptophan

* 9, 10

1617 cm −1 ν(C a C b ), observed in the spectra

of single human RBC

* 22

1618 cm −1 ν(C= C), tryptophan (protein

assignment)

* 20

ν(C= C), porphyrin * 20

Tryptophan * 20

Bound and free NADH * 54

Ring C-C stretch of phenyl(2) * 6

1620 cm −1 Peak of nucleic acids due to the

base carbonyl stretching and
ring breathing mode

* 50

ν(C= C), porphyrin * 33

Haemoglobin * 60

1620-40 cm −1 Bending vibration of R-CONHR

group

* 30

1620-750 cm −1 In-plane double end vibrations of

bases. The spectra in this region
are very sensitive to base-
pairing interactions and
base-stacking effects, i.e., effects
of hydrogen bond formation.

* 24, 30

1622 cm −1 Tryptophan * 3

Tryptophan and/or β-sheet * 3

Tryptophan (IgG?) * 43

1628 cm −1 C α = C α stretch * 6