Introduction to Spectroscopy - Vibrational Spectroscopy for Tissue Analysis - page 7

Laser source

Spectograph

Computer

Laser beam

Filter

Raman beam

CCD Camera

Optical microscope

Sample

Figure 1.5
Raman spectroscopy, a schematic diagram representing positioning of the necessary parts of
a Raman spectrometer.

Symmetrical stretching Anti-symmetrical stretching

Scissoring Rocking Wagging Tw isting

Figure 1.6
Different types of vibration of the chemical bonds.

the bands become unstable. In another four types, the length remains stable
and the angulations of the bands changes.

infrared (ir) Spectroscopy

Infrared spectroscopy has emerged as a powerful tool for chemical analysis
because of its ability to provide detailed information on the spatial distribu-
tion of chemical composition at the molecular level [23,24]. In applications