FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 247

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

1235 cm −1 Composed of amide III as well as

phosphate vibration of nucleic
acids

* 50

CH 6,2',α,α' rock * 6

Amide III * 7

In-phase ring, C-C, C-N-C stretch * 14

1236 cm −1 Amide III and asymmetric

phosphodiester stretching mode
(ν as PO 2 − ), mainly from the
nucleic acids

* 83

ν as PO 2 − of nucleic acids * 51

1236-42 cm −1 Relatively specific for collagen

and nucleic acids

* 40

1236/7 cm −1 Stretching PO 2 − asymmetric

(phosphate I)

* 48

1237 cm −1 Amide III and CH 2 wagging

vibrations from glycine
backbone and proline side chains

* 24

PO 2 − asymmetric (phosphate I) * 48

PO 2 − asymmetric * 88

1238 cm −1 Stretching PO 2 − asymmetric

(phosphate I)

* 48

Asymmetric phosphate
[PO 2 − (asym.)] stretching modes

* 64

Stretching PO 2 − asymmetric * 23

Amide III * 50

1238/9 cm −1 Asymmetric PO 2 − stretching * 78

1239 cm −1 Amide III * 15

1240 cm −1 One of the two most distinct

peaks for RNA (with 813 cm −1 )

* 12

Differences in collagen content * 24

* 38, 66

ν as PO 2 −

Collagen * 51

Asymmetric nonhydrogen-bonded
phosphate stretching mode

* 51

Asymmetric phosphate [PO 2 −
(asym.)] stretching modes
(Phosphate stretching modes
originate from the
phosphodiester groups of
nucleic acids and suggest an
increase in the nucleic acids in
the malignant tissues.)

* * 10

66

( Continued )