FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 246

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

1216 cm −1 Stretching of C-N * 28

1217 cm −1 PO 2 − asymmetric (phosphate I) * 48

1220 cm −1 PO 2 − asymmetric vibrations of

nucleic acids when it is highly
hydrogen-bonded

* 64

Asymmetric hydrogen-bonded
phosphate stretching mode

* 51

C= N= C stretching * 28

Amide III (β-sheet) * 16

1220-1 cm −1 Amide III (β-sheet) * 9

1220-4 cm −1 Phosphate II (stretching

PO 2 − asymmetric vibration) in
B -form DNA

* 23

1220-50 cm −1 νPO 2 − * 76

1220-300 cm −1 Amide III (arising from coupling

of C-N stretching and N-H
bonding; can be mixed with
vibrations of side chains)
(protein band)

* 29

Amide III

* 30, 60

1222 cm −1 Phosphate stretching bands from

phosphodiester groups of
cellular nucleic acids

* 51

CH 6,2',α,α' rock * 6

1222/3 cm −1 PO 2 − asymmetric (phosphate I) * 20, 48

1223 cm −1 ν(PO 2 − ), nucleic acids * 20

Cellular nucleic acids * 20

A concerted ring mode * 5

Proteins, including collagen I * * 8

1224 cm −1 Amide III (β sheet structure) * 43

1226 cm −1 PO 2 − asymmetric (phosphate I) * 48

1230 cm −1 Antisymmetric phosphate

stretching vibration

* 24, 30

Stretching PO 2 − asymmetric * 23, 50

Overlapping of the protein amide
III and the nucleic acid
phosphate vibration

* 50

1230-300 cm −1 Amide III (arising from coupling

of C-N stretching and N-H
bonding; can be mixed with
vibrations of side chains)

* 24, 30

1234 cm −1 A concerted ring mode * 5