FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 227

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

Hydroxyapatite * 9

968 cm −1 Lipids * 24

δ(= CH) wagging * 17

970 cm −1 Phosphate monoester groups of

phosphorylated proteins and
cellular nucleic acids

* 24

Symmetric stretching mode of
dianionic phosphate monoesters
of phosphorylated proteins or
cellular nucleic acids

* 52

DNA * 53

δ(= CH) wagging * 17

971 cm −1 νPO 4 = of nucleic acids and

proteins

* 51

Symmetric stretching mode of
dianionic phosphate monoester
in phosphorylated proteins,
such as phosvitin

* 52

* 51

ν(C-C) wagging * 43

972 cm −1 OCH 3 (polysaccharides, pectin) * 7

972/3 cm −1 C-C backbone (collagen

assignment)

* 19

973 cm −1 ρ(CH 3 ), δ(CCH) olefinic (protein

assignment)

* 33

974 cm −1 Ribose vibration, one of the

distinct RNA modes (with 874
and 915 cm −1 )

* 12

978 cm −1 CH 2,6' out-of-plane bending * 6

985 cm −1 OCH 3 (polysaccharides-cellulose) * 7

990-1010 cm −1 Ring stretch modes of benzene

derivatives

* 14

994 cm −1 C-O ribose, C-C * 48

995 cm −1 Ring breathing * 6

996 cm −1 Carbohydrates peak for solutions

and solids

* 24

C-O ribose, C-C * 48

Carbohydrates (weak shoulder) * 30

999 cm −1 ν 45 (CC), observed in the spectra

of single human RBC

* 18

1000 cm −1 Phenylalanine * 54

Bound and free NADH * 54

( Continued )