Biomedical Engineering Reference
In-Depth Information
Table 8.1 (
Continued
)
Major Characteristic
Peak Frequencies Reported in the Literature
Reference
Number
Peak
Assignment
FT-IR
Raman
782 cm
−1
DNA
*
8
Ring deformation, cyclic
deformation, C-H twist
*
14
U, C, T ring breathing, DNA
*
17
784 cm
−1
Phosphodiester; cytosine
*
21
U, C, T ring breathing, DNA
*
17
785 cm
−1
U, T, C (ring breathing modes in
the DNA/RNA bases)
*
12
Backbone O-P-O
Phosphodiester; cytosine
*
21
786 cm
−1
DNA: O-P-O, cytosine, uracil,
thymine
9
Pyrimidine ring breathing mode
*
4
787 cm
−1
Can be taken as a measure of the
relative quantity of nucleic acids
present
*
22
Phosphatidylserine
*
2
788 cm
−1
DNA:O-P-O backbone
stretching/thymine/cytosine
*
16
789 cm
−1
CH
α, α'
wagging and CH
6',6
out-of-plane bending and ring
puckering
*
6
792 cm
−1
CH
α, α'
wagging and CH
2',5',6'
out-of-plane bending and ring
puckering
*
6
793 cm
−1
Guanine in a
C
3
'endo/syn
conformation in the
Z
conformation of DNA
*
23
794 cm
−1
CH
α, α'
wagging and CH
2',5',6'
out-of-plane bending and ring
puckering
*
6
800-1200 cm
−1
Backbone geometry and
phosphate ion interactions
*
12
802 cm
−1
Uracil-based ring breathing mode
*
5
802-5 cm
−1
Left-handed helix DNA (
Z
form)
*
23
805 cm
−1
C
3
'
endo/anti
(A-form helix)
conformation
*
23
810/2 cm
−1
Phosphodiester (Z-marker)
*
21
813 cm
−1
Ring CH deformation
*
6
One of the two most distinct
peaks for RNA (with 1240 cm
−1
)
*
12
813 cm
−1
C-C stretching (collagen
assignment)
*
19
(
Continued
)
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