Chemical Structural Analysis of Bone by Spectroscopy - Vibrational Spectroscopy for Tissue Analysis - page 194

Table 7.1
Raman Peak Positions (cm −1 ) for Normal (Untreated) and Deproteinated Bone Tissue, Synthetic Hydroxyapatite, Calcium Carbonate,
Calcium Phosphate, Calcium Hydroxide, and Sodium Hydroxide with Assignments for Peaks (With permission.)

Normal Bone Deproteinated Bone Synthetic

Hydroxy
lapatite
(HA) P120

Standard Spectra

Human
Femur

Sheep
Femur

Human
Femur

Sheep
Femur

Bovine
Bone

Baboon
Bone

Calcium
Carbonate

Calcium
Phosphate

Calcium
Hydroxide

Sodium
Hydroxide Peak Assignment

CH stretch 3000-2900 3000-2900 2915 2915 2916 2916

Amide I band 1660 1660 1636 1637 1639 1639

CO 3 2− 1747

CO 3 2− 1434

C-H (bend) 1450 1450 1433 1434 1435 1435

Amide II band 1262 1262 1261 1261 1261 1262

C-O 1242 1242 1240 1240 1241 1241

CO 3 2− 1084

OH − 931 1079

CO 3 2− (stretch) 1062 1062 1062 1062 1062 1062

CO 3 2− (bend) 1036 1036 1036 1036 1036 1036 1045

PO 4 3− (symmetric
stretch)

952 952 952 952 952 952 952 952

OH − 801 373

721

PO 4 3− (asymmetric
stretch)

774
690

773

PO 4 3 − (bend) 584 585 584 584 585 585 706

CO 3 2− (bend) 710

PO 4 3− (bend) 429 429 429 429 429 429 436 431

OH − 355 293

249 207