Detecting and Monitoring of Cancer at the Cellular Level - Vibrational Spectroscopy for Tissue Analysis - page 177

1: CH region
2: Amide I
3: C=C (Protein Assignment)
4: CH 2 /CH 3 (Proteins + Phospholips)
5: CH 3 CH 2 wagging (Nucleic acids + Proteins)
6: Amide III
7: Aromatic CO and CN
8: vs PO 2 - (Nucleic acids + Phospholipids)
9: C-O stretching (Carbohydrates)

1700
1600
1500
1400
1300
1200
1100
1000
900
800
700
600
500
400
300
200
100

11

7 8 9

13
12

10

6

5

4

10: vs (C-C) (Proteins)
11: p(CH 2 /CH 3 ) + v(C-C) (Proteins)
12: v(C-C), δ(CCH) (Proteins + Polysaccharides)
13: O-P-O of DNA

2

3

: Gradual decrease in peak intensities

Red : A2780R-Control
Blue : A2789RCis-10umCDDP
Green : A2789RCis-20umCDDP
Purple : A2789RCis-30umCDDP

1

3000 2500 2000

Raman Shift (cm -1 )

1500 1000

Figure 6.8 (See colour insert.)
Changes in spectra of treated R samples.

13 1: CH region

2: Amide I
3: C=C (Protein Assignment)
4: CH 2 /CH 3 (Proteins + Phospholips)
5: CH 3 CH 2 wagging (Nucleic acids + Proteins)
6: Amide III
7: Aromatic CO and CN
8: vs PO 2 - (Nucleic acids + Phospholipids)
9: C-O stretching (Carbohydrates)

1100
1000
900
800
700
600
500

11 12

8 9

6

4

7

5 10

2

3

10: vs (C-C) (Proteins)
11: p(CH 2 /CH 2 ) + v(C-C) (Proteins)
12: v(C-C), δ(CCH) (Proteins + Polysaccharides)
13: O-P-O of DNA

: Sudden decrease in peak intensities

400
300
200
100
0
3000

Pink : A2780S-Control
Red : A2789SCis-0.3umCDDP
Blue : A2789SCis-1umCDDP
Green : A2789SCis-3umCDDP

1

2500 2000

Raman Shift (cm -1 )

1500 1000

Figure 6.9 (See colour insert.)
Changes in spectra of treated S samples.

of cisplatin and the resulted spectral changes, increasing the concentration
of the medication does not increase the modification dramatically. This con-
dition can be observed in the ~780, 1000-1100, and 1200-1400 cm −1 spectral
regions.
The CH region: Figure 6.13 clearly shows the significant change in the peak
intensities of CH bonds resulting from the application of cisplatin, and