Detecting and Monitoring of Cancer at the Cellular Level - Vibrational Spectroscopy for Tissue Analysis - page 174

Table 6.5
Peak Assignments and Definitions of Treated Cell Lines

Peak Position (cm −1 ) Assignment

483 Phosphate (Nucleic acids + phospholipids)

780 O-P-O of DNA

862 ν(C-C), δ(CCH) (Proteins + polysaccharides)

890 ρ(CH 2 ) + ν(C-C) (Proteins (proline, valine, tryptophan))

940 ρ(CH 3 ) + ν(C-C) (Proteins (proline, valine, keratin))

1013 ν s (C-C) (Proteins (phenylalanine))

1050 C-O stretching (Carbohydrates)

1090 ν s PO 2 − (Nucleic acids + phospholipids)

1200 Aromatic CO and CN

1245 ν(CN), δ(NH) (Proteins (amide III, collagen, tryptophan))

1325 CH 3 CH 2 wagging (Nucleic acids + proteins)

1450 δ(CH 2 ) δ(CH 3 ) (Proteins + phospholipids)

1550 ν(C=C) (Tryptophan and porphyrin, protein assignment)

1670 ν(C=O) (Proteins (amide I, collagen, elastin))

2930 CH region (Nucleic acids + proteins + lipids)

Apart from these general findings, there are some equally interesting and
more specific findings, which are described in the following text.

Spectra of R Samples

The fingerprint region: In the fingerprint region of spectra of the treated
R samples, the peaks flatten gradually with an increase in the drug concen-
tration. This finding can be seen in Figure 6.10. However, a relatively different
condition is seen in two areas:

a. In the amide I region, a shoulder is being added to the main peak
along with the increase in the strength of the medication.

b. A similar observation can be seen in the C = C peak (~1550 cm −1 ).

Both of these findings indicate creation of new chemical groups in the cells
as a result of the medication.
The CH region: The decrease in the peak intensities can obviously be seen
(Figure 6.11).

Spectra of S Samples

The fingerprint region: The sudden fall of the peak intensities and the collapse of
the overall structure of the spectra can be seen in Figure 6.12. In other words,
while in the R spectra, application of stronger medications will result in a
step-by-step fall in the peaks, in the S spectra, after applying the lowest strength