Detecting and Monitoring of Cancer at the Cellular Level - Vibrational Spectroscopy for Tissue Analysis - page 172

Table 6.4
Analysis of Cell Lines Treated with Different Concentrations
of cisplatin

A2780 R Samples A2780 S Samples

Control samples (A2780R Control) Control samples (A2780S Control)

A2789RCis-10 μm CDDP A2789SCis-0.3 μm CDDP

A2789RCis-20 μm CDDP A2789SCis-1 μm CDDP

A2789RCis-30 μm CDDP A2789SCis-3 μm CDDP

after preparation of normal cells. A total of eight samples were analysed.
Table 6.4, illustrates the details of the analysed samples. Each sample was
treated with the medication for 24 hours.
Five spectra were collected from each sample, except for A2789SCis-3 μm
CDDP, with three spectra (two of the spectra were not of appropriate quality
and were not useable).

Spectral analysis

To begin with, the spectra of control cell lines (not treated) are shown in
Figure 6.5. This figure also contains a summary of peak definitions and
assignments. The two separate images show the distinct spectral differ-
ences in both the CH and fingerprint areas. Peak definitions are tabulated
in Table 6.5.
Having a general look at the spectra, it can be concluded that the treated
S samples do not show the desirable reproducibility that is seen in the R
samples, particularly those treated with 1- and 3-μm drugs. This means
that the reactions of the S samples that have been treated with cisplatin
are not as constant as those of the R samples. This can be justified by
the fact that these cell lines are sensitive to chemotherapy, and their reac-
tions to treatment are not predictable. A similar finding can be seen only
in 30-μm spectra of the R samples. In other words, in low concentration
of cisplatin, R cell lines undergo a relatively constant modification as a
result of the drug, but as the concentration of the medication increases, the
modifications do not follow the previous constant pattern, and show some
inconsistencies.
Figures 6.6 and 6.7 illustrate the spectra of all R and S samples in one
image, respectively. It seems that spectral changes of the R samples follow
a gradual pattern, consistent with the gradual increase in the drug strength
(Figure 6.8). However, in the S samples, while a relatively significant dissimi-
larity can be observed between the control and 0.3-μm sample, the differ-
ences between the latter and the 1-μm and 3-μm samples are not that strong
(Figure 6.9). This means that due to the sensitive nature of the S samples, the
lowest concentration of the medication affects them considerably and higher
concentrations do not increase this effect in a constant way.