Biomedical Engineering Reference
In-Depth Information
6
Detecting and Monitoring of
Cancer at th
e Cellular Level
Introduction
Spectroscopy can be employed to study cancer cell lines. Raman spectroscopy
has been applied to analyse the testicular, lymphoma, and ovarian cancer
cell lines. In this chapter, a study describing the spectral differences between
resistant and sensitive subtypes of the 833k testicular cancer cell line sam-
ples is reported. Raman spectroscopy allowed reproducible and quantitative
analysis of the specimen and illustrated the chemical specifications of the
samples precisely. Six pairs of 833k testicular cancer cell lines were studied
and the findings were backed by statistical methods, i.e., partial least squares
discriminant analysis (PLS-DA). In addition, two other testicular cancer cell
lines (GCT27 and Susa), a lymphoma cell line (DHL4), and an ovarian cancer
cell line (A2789) treated with chemotherapeutic medications are analysed.
Raman spectroscopy can objectively differentiate between resistant and
sensitive cell lines. It can also analyse the effect of chemotherapeutic medica-
tions on cancer cell lines, as well as identify spectral differences between cell
lines of different organs. These results suggested that in the future it may be
possible to use cell lines and diagnostic Raman spectroscopy for preopera-
tive classification of biological molecules.
Analysis of Resistant and Sensitive Subtypes
of Testicular Cancer Cell Lines
Raman spectroscopy is an established technique for analysis of human tissue
samples, and several research groups have focused on this newly emerging
field [1-4]. However, most of the available literature is on applications of the
technique on human tissue samples. A few articles have used cancer cell
lines for spectroscopic investigations [5-7], and this field is relatively new in
spectroscopy.
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