Infrared and Raman Spectroscopy and Imaging of Breast Cancer - Vibrational Spectroscopy for Tissue Analysis - page 124

FTIR Differences among IDC Grades (I, II, and III)

The most dramatic changes in the FTIR spectra of cancerous tissues occur
in the spectral range of 1100 to 1000 cm −1 (phosphodiester stretching bands
region) [48,49] and show significant differences between the spectra of IDC
grades, as peak positioning is shifted in addition to changes in absorbance of
the peaks (Figure 5.13, outlined, boxed area). This indicates that the chemical

0.5

A

0.4

0.3

0.2

0.1

0.0

1800 1600 1400

Wavenumber, cm -1

1200 1000 800

0.5

B

1640 1630

1542

0.4

1522

0.3

0.2

0.1

0.0

1700 1600

Wavenumber, cm -1

1500 1400

Figure 5.13 (See colour insert.)
A: FTIR differences among IDC grades (I, in black; II in blue; and III, in red). B: Peak shifting in
protein amide I and II regions.