Biomedical Engineering Reference
In-Depth Information
Other Spectroscopic Studies
A summary of some interesting samples investigated by vibrational spectro-
scopic methods is provided in TableĀ 4.15. Characterisation of conformational
changes on guanine-cytosine and adenine-thymine oligonucleotides induced
by amiooxy analogues of spermidine using Raman spectroscopy was accom-
plished by A. J. Ruiz-Chica et al. These analogues resulted from the substitu-
tion of the two terminal aminomethylene groups of spermidine, + NH 3 CH 2 ,
by an amnooxy one, H 2 NO. The spectra demonstrated the existence of strong
differences in the oligonucleotide-analogue interactions depending on base
sequences. Different spectral features were observed. This fact supported the
idea that the two amino terminal groups of spermidine could have different
roles in the interaction of this biogenic polyamine with DNA [170].
K. J. Jalkanen et al. used vibrational spectroscopy to study protein and
DNA structure, hydration, and binding of biomolecules, as a combined
theoretical and experimental approach. The systems studied systemati-
cally were the amino acids, peptides, and a variety of small molecules. The
goal was to interpret the experimentally measured vibrational spectra for
these molecules to the greatest extent possible, and to understand the struc-
ture, function, and electronic properties of these molecules in their various
environments. It was also believed that the application of different spectro-
scopic methods to biophysical and environmental assays is expanding, and
therefore a true understanding of the phenomenon from a rigorous theoreti-
cal basis is required [171].
TableĀ 4.15
Articles on Different Samples
Research
Method
Investigated Tissue
or Sample
Effectiveness of
the Technique
Reference
Number
Research Group
Ruiz-Chika et al.
Raman
DNA
oligonucleotides
n/a
170
Jalkanen et al.
Raman & FTIR
DNA
n/a
171
Vo-Dinh et al.
Raman
Cancer genes
+
172
Binoy et al.
Raman & FTIR
Anticancer drug
+
173
Faolin et al.
Raman & FTIR
Tissue processing
n/a
174
Sokolov et al.
Reflectance
spectroscopy
Precancerous
lesions
n/a
175
Chen et al.
Raman
Fluorescent agents
+
176
Sahu and
Mordechai
FTIR
Cancer detection
+
177
Viehoever et al.
Raman
Organotypic raft
cultures
+
178
Rabah et al.
Raman
Neuroblastoma and
ganglioneuroma
+
179
 
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