Biomedical Engineering Reference
In-Depth Information
Table 4.11
Articles on Prostate Tissue
Research
Method
Investigated
Tissue or Sample
Effectiveness of
the Technique
Reference
Number
Research Group
Gazi et al.
FTIR
Prostate
+
150
Paluszkiewicz
and Kwiatek
FTIR and SRIXE
Prostate
+
151
samples of paraffin-embedded benign and cancerous prostate tissue. They
also successfully differentiated the prostate cancer cell lines derived from
different metastatic sites by using FTIR spectroscopy. It was found that the
ratio of peak areas of 1030 and 1080 cm −1 (corresponding to glycogen and
phosphate vibrations, respectively) suggests a potential method for the dif-
ferentiation of benign from malignant cells. It should be mentioned that
the tissues were analysed after mounting onto a BaF 2 plate and subsequent
removal of wax using Citroclear followed by acetone [150].
Paluszkiewicz and Kwiatek reported on human cancer prostate tis-
sues using FTIR microspectroscopy and synchrotron radiation induced
x-ray emission (SRIXE) techniques. The tissue samples were also analysed
by a histopathologist. In this research, differences between cancerous
and noncancerous parts of the analysed tissues were observed for both
m e t ho d s [151].
Colon Cancer
Colon, as a part of the gastrointestinal canal, has also been analysed spectro-
scopically (Table 4.12). The FTIR microspectroscopy study of S. Argov et al.
concerned inflammatory bowel disease as an intermediate stage between
normal and cancer. In this work, FTIR microspectroscopy was used to eval-
uate inflammatory bowel disease (IBD) cases and to study the IR spectral
characteristics with respect to cancer and normal tissues from formalin-
fixed colonic biopsies from patients. IBD tissues could be segregated from
cancer or normal ones using certain parameters such as phosphate content
and RNA/DNA ratios. The results exhibited that FTIR microspectroscopy
can detect biochemical changes in morphologically identical IBD and cancer
tissues and suggest which cases of IBD may require further evaluation for
carcinogenesis [152].
T. Richter et al. used FTIR spectroscopy in combination with positron emis-
sion tomography to identify tumour tissues. Thin tissue sections of human
squamous carcinoma from hypopharynx and human colon adenocarcinoma
grown in nude mice were investigated. Tumour tissues were successfully
 
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