Agriculture Reference
In-Depth Information
authors (Gahl
et al
., 1991; Fuller, 1994) suggest
that most EAAs are used with a similar effi-
ciency in pig and rat. In the Goettingen ap-
proach, the slope (
bc
-1
) calculated assumes
that the dietary protein quality (
b
) is linearly
dependent on the concentration of the limit-
ing AA in the feed protein (
c
) (Samadi and
Liebert, 2008). However, in this approach the
calculated efficiencies (
bc
-1
) for each EAA
are different and would be responsible for the
differences in the estimation of the EAA ratio
by the two methods. Clearly, research is still
needed in these fields in order to clarify the
potential limitations of these methodologies.
Results of this study support the premise
that the dilution method can be used to esti-
mate the optimum EAA pattern. Although
this methodology relies on some assumptions
that need to be further clarified, fast growth of
animals and rigorous dietary formulation are
expected to improve the consistency of the
results. Comparing the two methods, the esti-
mation of optimum amino acid ratio by the
Goettingen approach gave values closer to
those in the literature and also exhibited lower
variation.
Acknowledgements
The authors gratefully acknowledge the
financial support of Fundação de Amparo à
Pesquisa do Estado de São Paulo (FAPESP)
and the crystalline amino acids donated by
Ajinomoto LTDA.
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