Agriculture Reference
In-Depth Information
Table 15.3. Mean (± sd ) amino acid intake ( AAI ) and nitrogen balance ( NB ) of methionine + cystine
(Met+Cys), threonine (Thr), lysine (Lys) and valine (Val) trials with Bovans White (BovW) and Cobb genotypes .
Treatment
1
2
7 a
3
4
5
6
AA/
strain
mg/kg 0.73 day
Met
+Cys
AAI
0.0 ± 0.0
39.5 ± 0.95
59.8 ± 1.50
84.8 ± 2.52
166 ± 3.84
334 ± 10.1
638 ± 12.1
BovW c
NB b
- 296 ± 102
- 162 ± 119
72.1 ± 80.3
-65.7 ± 174
334 ± 155
615 ± 159
1814 ± 124
Met
+Cys
AAI
0.00 ± 0.0
55.3 ± 2.07
76.2 ± 1.91
120 ± 4.05
201 ± 10.85
263 ± 10.46 316 ± 9.45
Cobb c
NB
- 230 ± 55.4
-97.7 ± 85.2
428 ± 46.2
215 ± 96
259 ± 69.1
674 ± 166
695 ± 224
Thr
AAI
0.0 ± 0.0
36.7 ± 0.70
96.4 ± 1.25
73.1 ± 1.58
144 ± 1.39
287 ± 5.89
577 ± 13
BovW c
NB
- 203 ± 36.8
-37.2 ± 109
342 ± 74.1
132 ± 73.9
393 ± 170
1182 ± 43.8 1620 ± 184
Thr
AAI
0.0 ± 0.0
38.2 ± 0.59
96.4 ± 1.25
74.9 ± 1.79
152 ± 3.22
292 ± 5.56
584 ± 5.34
BovW c
NB
-364 ± 186
- 130 ± 91.7
342 ± 74.1
117 ± 97.6
487 ± 88.8
509 ± 168
1306 ± 230
Thr
AAI
0.0 ± 0.0
52.6 ± 2.46
95.3 ± 2.49
212 ± 9.33
324 ± 3.1
416 ± 7.74
542 ± 15.9
Cobb c
NB
-305 ± 109
-415 ± 105
468 ± 83.2
51.3 ± 144
597 ± 209
514 ± 269
698 ± 196
Lys
AAI
0.0 ± 0.0
88.0 ± 10.5
132 ± 10.6
176 ± 14.9
244 ± 15.9
338 ± 17.5
-
BovW d
NB
-387 ± 135
120 ± 8.92
139 ± 19
313 ± 34.1
635 ± 32.7
794 ± 58.3
-
Val
AAI
0.0 ± 0.0
91.8 ± 45.5
218 ± 19.8
222 ± 13.8
329 ± 33.4
439 ± 42.2
553 ± 64.8
Cobb e
NB
-548 ± 260
- 171 ± 94.9
- 157 ± 134
-47.7 ± 224
346 ± 81.5
493 ± 112
761 ± 138
a Additional level to verify the limiting amino acid in each trial.
b Nitrogen balance = nitrogen intake - nitrogen excretion. Nitrogen intake includes the consumption of a N-free diet offered
ad libitum .
c Bonato et al . (2011).
d Data not published.
e Data not published.
Discussion
In order to obtain the different scales
used to describe the maintenance esti-
mates, nitrogen and amino acid intake data
were transformed into their respective
scales based on bird weight (actual, meta-
bolic and protein weights). Considering
that amino acids are required only for pro-
tein, and not for water, fat or mineral main-
tenance, body protein content (mg/ BP 0.73 day)
was considered to be the most accurate. Figures
15.1 -15.4 show the graphical representation
of amino acid intake as a function of NB and
the regression equations (equation parameter
± standard deviation) on body protein scale
(mg/ BP 0.73 day).
The results obtained in the N balance trials
based on the methodology described by Non-
is and Gous (2008) showed consistent data,
and the scales used helped to improve the
accuracy of the requirement estimates. Be-
cause maintenance is a state in which the
animal is in nitrogen balance; that is, nitro-
gen intake is  equal to the sum of nitrogen
losses, thereby maintaining a constant nitro-
gen content in the body (Fuller et al ., 1976),
amino acids should be supplied at the same
rate as which they are lost (Sakomura and
Rostagno, 2007).
 
 
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