Biomedical Engineering Reference
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(a)
(b)
2 μm
2 μm
(c)
2 μm
FIGURE 1.7
(a) Apatite mineralization on the surface of nanosized MBG particles, (b) large-sized MBG
beads, and (c) porous MBG scaffolds.
1.4.2
In Vitro
Cell Response to MBG
MBG has been regarded as a potential bioactive bone regeneration mate-
rial due to its superior bioactive behavior. Further
in vitro
cell response
to MBG materials has been studied. We compared the attachment and
viability of human osteoblasts on four-composition MBG scaffolds and
found that MBG scaffolds with the composition of Si80Ca15P5 had the
best cell attachment (Zhu et al. 2008). MBG also supports the proliferation
of human Saos-2 osteoblasts, murine L929 fibroblasts, and murine SR.D10
T lymphocytes (Alcaide et al. 2010). The incorporation of MBG particles
into PLGA films enhanced the proliferation and ALP activity of human
osteoblasts (Wu, Ramaswamy, et al. 2009). Our study further showed that
silk modification of MBG scaffolds significantly improved the attach-
ment, proliferation, differentiation, and osteogenic gene expression of
bone marrow stromal cells (Wu, Zhang, Zhu, et al. 2010). In addition, the
incorporation of Fe, Sr, B, and Zr ions into MBG scaffolds could not only
support the attachment of BMSCs (Figure 1.8), but also enhance cell prolif-
eration and osteogenic differentiation (Wu, Fan, Gelinsky, et al. 2011; Wu,
Fan, Zhu, et al. 2011; Wu, Miron, et al. 2011; Zhu, Zhang, Wu, et al. 2011).
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